Thermostable lipase from Pichia sp. strain RT: Identification, production and characterization
نویسندگان
چکیده
منابع مشابه
Cloning, Expression and Characterization of a Thermostable Esterase HydS14 from Actinomadura sp. Strain S14 in Pichia pastoris
A thermostable esterase gene (hydS14) was cloned from an Actinomadura sp. S14 gene library. The gene is 777 bp in length and encodes a polypeptide of 258 amino acid residues with no signal peptide, no N-glycosylation site and a predicted molecular mass of 26,604 Da. The encoded protein contains the pentapeptide motif (GYSLG) and catalytic triad (Ser88-Asp208-His235) of the esterase/lipase super...
متن کاملPRODUCTION, RELEASE AND THERMAL CHARACTERIZATION OF CELLULOLYTIC ENZYME FROM CELLULOMONAS sp. STRAIN "0"
Cellulase production by a Cellulomonas sp., isolated in 1985 from forest humus soil along the border of the Caspian Sea in Iran, was investigated. This strain secreted endo-and exo-cellulases in the culture medium, but some of the enzymes produced remained cell membrane bound. Cell bound enzymes were released by various treatments. The highest amount of endo-glucanase (up to 95%) and exo-gl...
متن کاملProduction and characterization of lipase from Staphylococcus sp. SDMlip
Lipases (triacylglycerol hydrolases, EC 3.1.1.3) are enzymes that hydrolyze triacylglycerol at the oil-water interface and liberate free fatty acids and glycerol (Dheeman et al., 2010). Lipases belong to the class of serine hydrolases (Sharma et al., 2009) and contains the consensus sequence G-X1-S-X2-G as the catalytic moiety, where G = glycine, S = serine, X1 = histidine and X2 = glutamic or ...
متن کاملIsolation and Partial Characterization of a Bacterial Thermostable Polymethyl Galacturonase from a Newly Isolated Bacillus sp. strain BR1390
Background: Pectinases are pectin degrading class of enzymes including polygalacturonase (PG), polymethyl galacturonase (PMG), pectate lyase (PEL), and pectin esterase (PE) that are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juices. Objectives: A highly methylated pectin degrading bacterium from soil covered with fruit wa...
متن کاملOptimization of physical conditions for the production of thermostable T1 lipase in Pichia guilliermondii strain SO using response surface methodology
BACKGROUND Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the screening and optimisation of physical conditions for T1 lipase production in P. guilliermondii. RESULT The screening of six physical condit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Asia Pacific Journal of Molecular Biology and Biotechnology
سال: 2018
ISSN: 2672-7277
DOI: 10.35118/apjmbb.2018.026.1.03